Do you mind sharing your tricks? Do you use bleach?I found Sarr. seed fairly easy in vitro.
Thanks Willy, At first I had quite a time finding a source but I believe you can still get 30 grams for roughly 30 dollars plus shipping at 35 dollars from:Great work, Kent!
Where did you buy your PPM? It is so expensive..
I adjust my ph to 5.8 before I put the jars into the pressure cooker.
Your recipe looks like a few others Ive seen except for the multi-vitamin tablet.@Dogrem, thanks! Most people refer this to TC, but I guess the correct term is in vitro, haha.
@housepet
Thanks for the recipe!
This is mine (haven't added anything else yet, but I bought some BAP):
1/3 MS salts
30g table sugar
1/8 tablet of multi-vitamin tablet with thiamine
6g food grade agar agar
On a side note, I usually mix half a liter at a time.
I havent tried anything other than Dionaea so far because my big problem is always fungus on the tips of ebay seed even after 10 percent bleach for ten minutes. The only change lately is that I just started using tween 20 in the bleach bath before rinsing with tap water 5 minutes through a tea strainer. As an added precaution, I let them soak a few hours in sterile distilled water before sucking them out with a plastic pipette and then dropping them into jars. Even after all that, I will only get a few clean sprouts from each pack. My best average of 5 seeds without fungus came from an ebayer in Tisdale Sask.I found Sarr. seed fairly easy in vitro.
You had me fooled Willy. Judging by the amount of roots in this batch, I was certain you were using some magic formula.I've only bought BAP to play with. I haven't added any to my cultures yet, I want to get them growing into decent sizes before inducing the multiplication.
Here are some photos from my mini-lab:
Some Drosera seedlings...
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Byblis
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A transferred culture, hopefully nothing goes wrong!
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Sarracenia seed!
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For the 1/8 tablet, which vitamin is this?@Dogrem, thanks! Most people refer this to TC, but I guess the correct term is in vitro, haha.
@housepet
Thanks for the recipe!
This is mine (haven't added anything else yet, but I bought some BAP):
1/3 MS salts
30g table sugar
1/8 tablet of multi-vitamin tablet with thiamine
6g food grade agar agar
On a side note, I usually mix half a liter at a time.
I have a few ex-plant cultures just made few days ago. Let's hope that something will happen (beside fluffy fungus growing).You had me fooled Willy. Judging by the amount of roots in this batch, I was certain you were using some magic formula.
Have you had any success from cuttings?